Bladder weight of BOO rats was significantly increased (0.35±0.041 g vs. 0.11±0.0048 g, respectively, p<0.0001) compared to sham rats. In CMG, BOO rats were significantly increased in the amplitude during voiding (91±6.5 cmH2O vs. 40±2.2 cmH2O, respectively, p<0.0001), a number of non-voiding contractions (0.91±0.15 /min vs. 0.11±0.025 /min, respectively, p=0.00020), post-void residual (1200±230 µl vs. 20±8.4 µl, respectively, p=0.0004), bladder capacity (2000±240 µl vs. 860±32 µl, respectively, p=0.0006), and compliance (0.89±0.17 ml/H2O vs. 0.31±0.032 ml/H2O, respectively, p=0.0051) compared to sham rats (figure 1). Voiding efficiency (VE) was decreased in BOO rats compared to sham rats (46±6.9 % vs. 98±0.89 %, respectively, p<0.0001). In sham rats, intravesical administration of CCPA, ZM241385 or inosine did not have any effect on CMG parameters. In BOO rats, CCPA and Inosine did not have any effect on CMG parameters; however, ZM241385 significantly reduced NVCs in BOO rats (0.50±0.079 /min vs. 0.90±0.15 /min, respectively, p=0.028) (figure 2). mRNA expression levels of adenosine A2A receptors and A3 receptors in bladder mucosa were significantly increased in BOO rats compared to sham rats (p<0.0001, p=0.0145, respectively). In contrast, mRNA expression levels of adenosine A2B receptors in bladder mucosa was significantly decreased in BOO rats compared to sham rats (p<0.0001). There was no significant difference in adenosine receptor expression in detrusor between BOO and sham rats.