Study design, materials and methods
We conducted a monocentric prospective study including non-pregnant, nulliparous women. Women with personal history of pelvic floor disorders, body mass index higher than 35Kg.m-2, muscular disease were excluded. Two visits were planned with a time interval ranged from 12h to 7 days. During each visit it was performed a measure of the elastic properties of the right LAM by a single investigator which is a senior Urogynecologist. These measurements were done during three conditions: rest, Valsalva maneuver and perineal contraction. For each measure, the participants lay down in the lithotomy position with an empty bladder. We used an Aixplorer ® device (V12, Supersonic Imagine, France) with a linear SL 18-5 probe (5-18 MHz). The pubic insertion of the right LAM was identified in B-mode with a transperineal approach and then we proceeded to perform the SWE acquisition. Before any measurement, the volunteer performed 2 Valsalva maneuvers with biofeedback to prevent LAM coactivation. For assessments at rest, the women had to relax as much as possible. For assessment during Valsalva, she had to perform a maximal Valsalva maneuver for at least 5s and for assessments at contraction she had to contract her perineum as if she wanted to avoid gas leakage for at least 5s. Data were collected as 5 seconds video clips with three consecutives video clips for each condition: rest, Valsalva and contraction [1,2]. Within each video clip the region of interest was identified and contoured using Matlab scripts (The Mathworks, Inc., 2016) within we measured the shear modulus (SM), in kPa. For measurements at rest and Valsalva maneuver, the mean SM during the video clip was considered whereas for measurement during contraction we considered the maximal SM modulus. For the primary objective, we analyzed the intersession reproducibility for each condition with the Intraclass Correlation Coefficient (ICC), the Standard Error of Measurement (SEM), and the coefficient of variation (CV) serving as main judgement criteria. For this analysis we considered the mean of the three consecutives measurements for each condition. To address our secondary objectives, we investigated the intrasession reproducibility within three consecutives measures using the same methods than for the primary objective. Last, we compared the reproducibility performances when considering the mean of the three consecutive measures or the first f the three measures. Statistical analysis was performed using STATA software (V14IC; Stata Corporation, College Station, TX, USA). Continuous variables were reported as means and standard deviation (SD). This study was approved by an ethical committee and each woman gave her written free consent before any investigation.
Interpretation of results
The reliability was excellent at rest and Valsalva maneuver when considering the mean of the three consecutives measures or the first one. This result would have been the same if considering the second or the third measure because the intrasession reproducibility was also excellent. Based on this observation, it appears safe to perform one single measure when the objective is to assess the elastic properties for a specific and unique time. If the objective is to investigate changes across the time, it is safer to perform three measures and consider their mean regarding that this seems increase the reliability. The possibility of safely measuring the elastic properties of women’s pelvic floor muscles in a non-invasive way is very innovative and allows some very interesting prospects. As an example, this technology is currently used in a prospective study in a pregnant women cohort and it is expected from this study to provide the first human data about changes in the elastic properties of the pelvic floor during pregnancy and their interactions with the risk of perineal trauma at childbirth [2, 3]. Likely, this technology offers the possibility to measure the elastic properties of the pelvic floor muscles in women suffering from pelvic floor disorders with the prospect of an individual discussion about the treatment strategy based on the patient’s intrinsic characteristics.